- Additional information
|Other Names||Long R3-IGF-1; IGF-1 Long R3|
|IUPAC Name||N/A |
|Purity||≥99% Pure (LC-MS)|
|Powder Availability||1 milligram, 10 milligrams (lyophilized/freeze-dried)|
|Storage||Store in a dry, cool, dark place. For best preservation, store at 4°C or colder away from bright light.|
|Terms||All products are for laboratory developmental research USE ONLY. Products are not for human consumption.|
**Important Information: Each peptide comes lyophilized/freeze-dried and must be reconstituted with Bacteriostatic Water in order to be dispensable in liquid form.
What is IGF-1 LR3
IGF-1 or Insulin-like Growth Factor is a peptide that is capable of managing and regulating levels of growth hormone (GH). IGF-1 LR3 is a lengthened version of IGF-1. IGF-1 LR3 is a chemically altered amino acid chain made of 83 amino acids. At position 3 on the chain Glu(E) was substituted with Arg(R), which gives the name LR3. By making this substitution at position three, IGF-1 is inhibited from binding to certain proteins in the body, thus increasing the half-life (https://www.prnewswire.com/news-releases/igf-1-and-long-igf-1-lr3-is-there-a-difference-us-peptides-discusses-190362251.html).
Effects of IGF-1 on Growth Hormone Secretion
IGF-1 is a peptide that acts similarly to insulin, and its main function in the body is to stimulate growth by increasing GH secretion from the pituitary gland, as well as regulating cell growth and cell death. The functioning of the pituitary gland and the hormones it secretes decreases with aging, however, IGF-1 is capable of bypassing the pituitary gland and is able to elicit the same response as GH does.
There have been extensive animal-based studies conducted on the functioning of IGF-1 in the body. As was previously mentioned, levels of GH and IGF-1 steadily decrease over a lifespan, and with the lower hormone levels comes a myriad of side effects such as increased fat mass, cardiovascular dysfunction, decreased muscle mass, and declined cellular protein synthesis. As GH is secreted in the body, it prompts the release of IGF-1 from the liver, which in turn regulates the growth effect throughout the body.
IGF-1 and Longevity
Research has shown that while decreased levels of GH and IGF-1 lead to the deterioration of various physiological functions, these decreased levels have also shown promise in increasing life span. Multiple studies have been conducted connecting deficiencies in these hormones to the length of lifespan and how different variables such as diet can further affect an animal’s lifespan.
For example, a recent study examined animals that had a significant mutation in the growth hormone releasing hormone receptors (GHRHR) which led to decreased levels of circulating GH. Due to the decrease in GH secretion, the secretion of IGF-1, which is driven by GH release, was also drastically reduced. The animals experiencing these deficiencies only grew up to ⅔ of the control subjects. However, the interesting part of this study mentioned how as the animals aged there was no clear reduction in longevity despite the obesity that developed, but when these animals were placed on a low-fat diet, lifespan increased by 30%. That being said, further research will be conducted regarding the correlation between the growth hormone-IGF-1 axis and the pathology of aging and overall longevity (https://academic.oup.com/biomedgerontology/article/57/5/B177/620569).
Peptides Prefer the Cold
In order to reduce peptide breakdown, keep peptides refrigerated at all times but DO NOT FREEZE.
Swab the top of the vial with a 95% alcohol wipe before accessing.
Only Mix with Sterile Bacteriostatic Water
Bacteriostatic water is vital to preventing contamination and preserving the stability of the compound.
Push the pin through the stopper at an angle in order to direct the stream to the side of the vial.
Reconstituted peptide solution should be stored at around 4 degrees Celsius but not frozen, while lyophilized peptide solution should be kept at -20 degrees Celsius.
Long arginine 3-IGF-1, abbreviated as IGF-1 LR3 or LR3-IGF-1, is a synthetic protein and lengthened analogue of human insulin-like growth factor 1 (IGF-1). It differs from native IGF-1 in that it possesses an arginine instead of a glutamic acid at the third position in its amino acid sequence (“arginine 3”), and also has an additional 13 amino acids at its N-terminus (MFPAMPLLSLFVN) (“long”), for a total of 83 amino acids (relative to the 70 of IGF-1). The consequences of these modifications are that IGF-1 LR3 retains the pharmacological activity of IGF-1 as an agonist of the IGF-1 receptor, has very low affinity for the insulin-like growth factor-binding proteins (IGFBPs), and has improved metabolic stability. As a result, it is approximately three times more potent than IGF-1, and possesses a significantly longer half-life of about 20–30 hours (relative to IGF-1’s half-life of about 12–15 hours).
IGF-1 LR3 Peptide is under active investigation in a number of cell culture and animal models.
Our IGF-1 LR3 Peptide is stored and handled according to industry guidelines to ensure stability.
PEPTIDES PREFER THE COLD
Keep peptide vials refrigerated at all times to reduce peptide bond breakdown. DO NOT FREEZE. Most peptides, especially shorter ones, can be preserved for weeks if careful.
Always swab the top of the vial with an alcohol wipe, rubbing alcohol or 95% ethanol before use.
Before drawing solution from any dissolved peptide vial, fill the pin with air to the same measurement you will be filling with solution, ie. if you plan to take 0.1 ml, first fill the pin with 0.1ml of air, push the air into the vial, and then draw the peptide back up to the 0.1 ml marker. Doing so will maintain even pressure in the vial. Always remember to remove air bubbles from the pin by flicking it gently, pin side up, and pushing bubbles out. In addition, push out a tiny amount of solution to ensure there is no air left in the metal tip.
ONLY MIX WITH STERILE BACTERIOSTATIC WATER
The purity and sterility of bacteriostatic water are essential to prevent contamination and to preserve the shelf-life of dissolved peptides.
Push the pin through the rubber stopper at a slight angle, so that you inject the bacteriostatic water toward the inside wall of the vial, not directly onto the powder.
Lyophilized peptide should be stored at -20°C (freezer), and the reconstituted peptide solution at 4°C (refrigerated). Do not freeze once reconstituted.
NEVER SHAKE A VIAL TO MIX.
Air bubbles are unfavorable to the stability of proteins.
**LAB USE ONLY**
*This information is for educational purposes only and does not constitute medical advice. THE PRODUCTS DESCRIBED HEREIN ARE FOR RESEARCH USE ONLY. All clinical research must be conducted with oversight from the appropriate Institutional Review Board (IRB). All preclinical research must be conducted with oversight from the appropriate Institutional Animal Care and Use Committee (IACUC) following the guidelines of the Animal Welfare Act (AWA).
IGF-1 LR3 is sold for laboratory research use only. Terms of sale apply. Not for human consumption, nor medical, veterinary, or household uses. Please familiarize yourself with our Terms & Conditions prior to ordering.